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How do you cut a TLC glass plate?
Using a sharp glass cutter and a ruler as a guide, you should have no problem scoring the glass. Once the entire plate is scored, you can then break the glass into individual pieces. Try to handle the plates by the edges to prevent contamination of the silica surface.
Why should you not touch the TLC plate?
Never under any circumstances touch the face of a TLC plate with your fingers as contamination from skin oils or residues on gloves can obscure results.
How to Cut TLC Plates
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What are the methods of preparation of TLC plates?
After preparing the slurry, the TLC plates can be prepared by using any one of the following techniques; pouring, dipping, spraying and spreading • Pouring technique: the slurry is prepared and poured on to a glass plate which is maintained on a leveled surface.
Are TLC plates glass?
TLC Glass Plates L × W 20 cm × 20 cm, silica gel 60 matrix, binder, Organic Polymer, fluorescent indicator | Sigma-Aldrich.
What are TLC plates coated with?
A TLC plate is a sheet of glass, metal, or plastic which is coated with a thin layer of a solid adsorbent (usually silica or alumina).
What happens if you touch the front of a TLC plate with your finger s?
What happens if you touch the front of a TLC plate with your finger(s)? After placing the TLC plate in the eluent, the eluent will travel up the TLC plate till it encounters the spot (compound).
At what height should you remove the TLC plate from the development tank?
Place the sample in the TLC chamber with forceps, cap it, and leave it alone. Remove the plate when the solvent line is ∼0.5cm from the top.
How do I select a solvent for TLC?
The most suitable solvent system is the one that moves all components off the baseline with Rf values between 0.15 and 0.85 (ideally, close to 0.2 – 0.4). Remember that it is not always possible in TLC but should be possible in flash chromatography where solvent gradients can be used.
Which method is best for the preparation of TLC plate?
The method is used in the preparation of TLC plates are Pouring, Spreading, Dipping.
When can a TLC plate be removed?
The developing chamber should have some developing solvent in it, but the level of this solvent should not be above the pencil marks and spots on your plate. Allow the solvent to climb up the TLC plate, and remove the plate when the solvent nearly reaches the top.
How would we get better separation between spots on a TLC plate?
Use pre-coated TLC plates. If you are using a single solvent as a mobile phase then try for combination of two or more than two solvents having differences in polarity. if you still failing in separation of spots then try different ratios of mobile and go on trial and error base to choose proper mobile phase.
Cutting TLC Plates
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How does TLC work polarity?
The eluting power of solvents increases with polarity. Therefore, low polarity compounds can be eluted with low polarity solvents, while higher polarity compounds require solvents of higher polarity. The stronger a compound is bound to the adsorbent , the slower it moves up the TLC plate.
What is a TLC spotter?
Capillary spotters, or TLC spotters, are very useful for depositing small amounts of sample onto a TLC (thin layer chromatography) plate.
How many types of TLC are there?
SiliCycle offers different types of plates for thin layer chromatography applications: classical TLC, high performance TLC (also called HPTLC) and preparative TLC (PLC). The plate types are selected based on the type of analysis required and the available budget.
Why do we mark TLC plates with pencil and not with pen?
Answers and Solutions
Why do you use a pencil and not a pen to mark TLC plates? Answer: The pen ink becomes mobile on the plate and travels up the TLC plate with TLC solvent. But the solid particles of graphite in the pencil won’t get dissolved and hence can be used to mark TLC plates.
Why is Binder used in TLC?
Gypsum functions as a binder, improving the cohesion of the adsorbent particles and increasing the adhesion of the layer to the glass substrate. These plates are made in the classic style and are thus softer than plates with organic binder.
What is a solvent front?
In chromatography, the solvent front is the position on the TLC plate indicating the furthest distance traveled by the developing solvent (or eluent)
How can you avoid contaminating a TLC plate?
…
- gather glassware, silica, sand, glass wool, solvent and sample prior to assembling the column.
- insert a very small (but not tightly packed) amount of glass wool right above stopcock and clamp column to the ring stand.
- fill column about 1/3 up with petroleum ether.
Can an Rf be greater than 1?
By definition, Rf values are always less than 1. An Rf value of 1 or too close to it means that the spot and the solvent front travel close together and is therefore unreliable.
What are the 4 types of chromatography?
- Types of chromatography. Column chromatography. …
- Column chromatography. …
- Ion- exchange chromatography. …
- Gel- permeation (molecular sieve) chromatography. …
- Affinity chromatography. …
- Paper chromatography. …
- Thin-layer chromatography. …
- Gas chromatography.
Cutting TLC Plates
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Which side of the TLC plate will you be spotting?
Spotting. The powdered side of the silica gel plate, not the shiny, plastic side is the side that is spotted. Lightly draw a pencil line about 1 cm from the end of a plate. Use a pencil, not a pen, and be careful to not scratch the surface of the plate too deeply.
What does it mean when there are multiple spots on a TLC plate?
Ideally, each compound in a mixture will produce a distinct spot so a sample with two compounds will give two different spots, and so on. An important property of any compound, is its Rf-value (retention factor). In simple terms, this value is an indication of how far up a TLC-plate a compound has wandered.
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